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Stability and O 2 -sensing characteristics of the Matrigel-based scaffolds impregnated with the different O 2 probes: ( A )—normalised phosphorescence intensity and lifetime signals of the Matrigel–MitoXpress and Matrigel–NanO2 scaffolds (5 mM probe), before and after 12 h exposure to DMEM medium at 37 °C (N = 3; t -test: p < 0.0005 and p < 0.001 for MitoXpress and NanO2, respectively); ( B , C )—phosphorescence intensity ( B ) and lifetime ( C ) signals of the Matrigel–NanO2 scaffold coatings (20 μM probe) before and after the 18 h exposure to DMEM medium at 37 °C. In ( B ), N = 3; t -test for differences between intensity signals at 0 h and 18 h: p = 0.15, p < 0.05 and p < 0.005 for 3 mL used sensor, 6 μL used sensor and 6 μL fresh sensor, respectively. In ( C ), N = 3, t -test for differences in lifetime signals: p > 0.5 for all samples. ( D )—O 2 calibration of the Matrigel–Nano-IR scaffold, generated by the barometric method at 21 °C.

Journal: Biosensors

Article Title: Hybrid Oxygen-Sensing Bio-Scaffolds for 3D Micro-Tissue Models

doi: 10.3390/bios16020122

Figure Lengend Snippet: Stability and O 2 -sensing characteristics of the Matrigel-based scaffolds impregnated with the different O 2 probes: ( A )—normalised phosphorescence intensity and lifetime signals of the Matrigel–MitoXpress and Matrigel–NanO2 scaffolds (5 mM probe), before and after 12 h exposure to DMEM medium at 37 °C (N = 3; t -test: p < 0.0005 and p < 0.001 for MitoXpress and NanO2, respectively); ( B , C )—phosphorescence intensity ( B ) and lifetime ( C ) signals of the Matrigel–NanO2 scaffold coatings (20 μM probe) before and after the 18 h exposure to DMEM medium at 37 °C. In ( B ), N = 3; t -test for differences between intensity signals at 0 h and 18 h: p = 0.15, p < 0.05 and p < 0.005 for 3 mL used sensor, 6 μL used sensor and 6 μL fresh sensor, respectively. In ( C ), N = 3, t -test for differences in lifetime signals: p > 0.5 for all samples. ( D )—O 2 calibration of the Matrigel–Nano-IR scaffold, generated by the barometric method at 21 °C.

Article Snippet: NanO2 and Nano-IR nanoprobes , Not obvious , Stable structures, no probe leakage, low toxicity, red-emitting probe , Animal cells, spheroids, organoids, tissue biopsies.

Techniques: Generated

Phosphorescence lifetime profiles of the HCT116 cell spheroids seeded in 6 mL of 50% Matrigel scaffold containing 1 mg/mL of NanO2 probe and covered with 100 mL of McCoy medium, measured on a Victor2 plate reader in TR-F mode. After temperature equilibration of the plate at 37 °C, 100 μL of oil was applied on top of the sample (at ~20 min) to limit back diffusion of O 2 from air.

Journal: Biosensors

Article Title: Hybrid Oxygen-Sensing Bio-Scaffolds for 3D Micro-Tissue Models

doi: 10.3390/bios16020122

Figure Lengend Snippet: Phosphorescence lifetime profiles of the HCT116 cell spheroids seeded in 6 mL of 50% Matrigel scaffold containing 1 mg/mL of NanO2 probe and covered with 100 mL of McCoy medium, measured on a Victor2 plate reader in TR-F mode. After temperature equilibration of the plate at 37 °C, 100 μL of oil was applied on top of the sample (at ~20 min) to limit back diffusion of O 2 from air.

Article Snippet: NanO2 and Nano-IR nanoprobes , Not obvious , Stable structures, no probe leakage, low toxicity, red-emitting probe , Animal cells, spheroids, organoids, tissue biopsies.

Techniques: Diffusion-based Assay